Journal: iScience

Article Title: Heme regulates protein interactions and phosphorylation of BACH2 intrinsically disordered region in humoral response

doi: 10.1016/j.isci.2024.111529

Figure Lengend Snippet:

Article Snippet: The primary antiserum and antibodies used were an anti-BACH2 anti-serum (BACH2-N2), and anti-TBK1/NAK (3504, Cell Signaling Technology), anti-DNMT1 (ab13537, Abcam), anti-GST (GE27–4577-01, Sigma Aldrich), anti-phospho-p70S6K (9205, Cell Signaling Technology), anti-p70S6K (9202, Cell Signaling Technology), anti-Lamin B (C-20) (sc-6216, Santa Cruz Biotechnology) and anti-GAPDH antibodies (ab8245, Abcam).

Techniques: Virus, Recombinant, Protease Inhibitor, Transfection, Multiplex sample analysis, Protein Purification, Reporter Assay, Luciferase, Cell Isolation, Plasmid Preparation, Reverse Transcription, SYBR Green Assay, ChIP-qPCR, Sequencing, Negative Control, Software

Journal: Cell chemical biology

Article Title: Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein

doi: 10.1016/j.chembiol.2019.04.001

Figure Lengend Snippet: A. Plot comparing mRNA level for high confidence ALLC interacting proteins (measured by RNAseq in (Plate et al., 2016); n=3 biological replicates) vs. their increased interactions with FTALLC in HEK293DAX cells following stress-independent ATF6 activation (n=7 biological replicates). The dashed line shows least-squares linear regression. The solid lines show 95% confidence intervals. B. Plot comparing cellular protein level for high confidence ALLC interacting proteins (measured by whole cell quantitative proteomics in (Plate et al., 2016); n=3 biological replicates) vs. their increased interactions with FTALLC in HEK293DAX cells following stress-independent ATF6 activation (n=7 biological replicates). The dashed line shows least-squares linear regression. The solid lines show 95% confidence intervals. C. Plot comparing cellular protein level for high confidence ALLC interacting proteins (measured by whole cell quantitative proteomics in (Plate et al., 2016); n=3 biological replicates) vs. their increased interactions with FTALLC in HEK293DAX cells following stress-independent XBP1s activation (n=7 biological replicates). The dashed line shows least-squares linear regression. The solid lines show 95% confidence intervals. D. Plot comparing cellular protein level for high confidence ALLC interacting proteins (measured by whole cell quantitative proteomics in (Plate et al., 2016); n=3 biological replicates) vs. their increased interactions with FTALLC in HEK293DAX cells following stress-independent ATF6 and XBP1s co-activation (n=4 biological replicates). The dashed line shows least-squares linear regression. The solid lines show 95% confidence intervals. E. Graph showing changes in protein levels (open symbols; n=3) or FTALLC interactions (solid bars; n=4-7) for PDIA4 in HEK293DAX cells following stress-independent XBP1s (red), ATF6 (blue), or XBP1s and ATF6 (green) activation. Error bars show SEM for the individual replicates. F. Graph showing changes in protein levels (open symbols; n=3) or FTALLC interactions (solid bars; n=4-7) for HYOU1 in HEK293DAX cells following stress-independent XBP1s (red), ATF6 (blue), or XBP1s and ATF6 (green) activation. Error bars show SEM for the individual replicates.

Article Snippet: PDIA4 (ERp72) (rabbit polyclonal) , ProteinTech , Cat#14712-1-AP.

Techniques: Activation Assay, Quantitative Proteomics

Journal: Cell chemical biology

Article Title: Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein

doi: 10.1016/j.chembiol.2019.04.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: PDIA4 (ERp72) (rabbit polyclonal) , ProteinTech , Cat#14712-1-AP.

Techniques: Virus, Recombinant, Sequencing, Modification, Purification, Labeling, Multiplex sample analysis, Expressing, Plasmid Preparation, Cloning, Software

Journal: iScience

Article Title: Heme regulates protein interactions and phosphorylation of BACH2 intrinsically disordered region in humoral response

doi: 10.1016/j.isci.2024.111529

Figure Lengend Snippet:

Article Snippet: The primary antiserum and antibodies used were an anti-BACH2 anti-serum (BACH2-N2), and anti-TBK1/NAK (3504, Cell Signaling Technology), anti-DNMT1 (ab13537, Abcam), anti-GST (GE27–4577-01, Sigma Aldrich), anti-phospho-p70S6K (9205, Cell Signaling Technology), anti-p70S6K (9202, Cell Signaling Technology), anti-Lamin B (C-20) (sc-6216, Santa Cruz Biotechnology) and anti-GAPDH antibodies (ab8245, Abcam).

Techniques: Virus, Recombinant, Protease Inhibitor, Transfection, Multiplex sample analysis, Protein Purification, Reporter Assay, Luciferase, Cell Isolation, Plasmid Preparation, Reverse Transcription, SYBR Green Assay, ChIP-qPCR, Sequencing, Negative Control, Software